Dr Seyed Amir Mousavi
Assistant Professor of Endodontics, Isfahan University of Medical Sciences
Dental pulp tissue has the potential to regenerate dentin in response to specific stimulations. Stem cell therapy might be considered as an available method in pulp dentin regeneration. The aim of this study was an in-vivo evaluation of deciduous pulp's stem cell in pulp and dentin regeneration. In order to isolate stem cells, 1 Iranian mixed-breed 6-months dog was used. four deciduous teeth were extracted. Pulp of the teeth were isolated and exposed to type 1 collagenase enzyme. Isolated cells were cultured on Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 10 % fetal bovine serum(FBS) and 1% antibiotic.
Although there was no significant difference between 3groups except for calcified barrier type between group 1 and 2, Dentin like matrix, collagen fibers and small vessels observed in the cavity on the amputated pulp after using stem cells.
The results of the study suggest the possibility of pulp and dentin regeneration with stem cells in damaged teeth.