Propolis Microgel As Protective Agent for Odontoblast Cell in External Bleaching
Hasanuddin University, Indonesia
Aim : To evaluate the effects of 10% propolis microgel (PM) on the MDPC-23 odontoblast cell line viability in external bleaching.
Materials and Methods: The cells were seeded in well plate with Dulbecco’s Modified Eagle Medium (DMEM) and incubated for 48 hours. Enamel dentin disc is placed in an acrylic transwell above the MDPC-23 odontoblasts cell line and it slightly contacted with the cells in the well plate. 40 % Hydrogen peroxide (H2O2) and 10% propolis microgel were applied over enamel-dentin disc during treatment. Six groups of cells (n=10) were treated as follows: P0: no treatment (control); P1: H2O2 /20 min; P2: H2O2 /20 min, flushed, incubated/30 min; P3: H2O2 /20 min, flushed, PM/30 min; P4: PM/30 min, flushed, H2O2 /20 min; and P5: PM/30 min. The cells viability were evaluated by MTT assay. The data obtained were analyzed by ANOVA test (p < 0.05).
Results : The percentages of cells viability were as follows: P0 (control)= 69.063%; P1=23.844%, P2=31.579 %, P3=50.964 %, P4=53.349 %, and P5=57.628 %. Group P3 and P4 presented a statistically higher cell viability than group P1 and P2. PM decreased cell death percentage caused by H2O2, demonstrating its protective effect against the toxic components of this bleaching agent.
Conclusions : It was concluded that 10% propolis microgel could maintain the viability of MDPC-23 odontoblastic cells, so it can be used to to protect these cells against the cytotoxic effects of H2O2.
Key words: Bleaching agent; Hydrogen peroxide; Odontoblasts; Microgel propolis; Cytotoxicity.